Immunohistochemistry / Immunofluorescence

Immunohistochemistry / Immunofluorescence


Immunohistochemistry (IHC) General protocol

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Protocol revelation amplified : DBS Range
I. Required material Reagents - Primary antibody - Primary Antibody Diluent - Pre-Blocking solution - Mounting medium Buffers - Immuno Wash Buffer - Xylene or dewaxing reagents - Absolute ethanol - Distilled or deionized water II. Experimentation length - 10 mn of blocking - 30-60 mn of sections incubation in primary antibody - 36 or 31 mn of others incubation - 20 mn of rinsing steps - TOTAL : 1h31 to 2h06 III. Staining procedure 1...