How would you measure the Telomere length ?

How would you measure the Telomere length ?

2 types of SYBR® Green qPCR-based assay kits for the accurate, simple and fast telomere length quantification for Human, Mouse and Rat samples

Telomeres are repetitive nucleotide elements at the ends of chromosomes that protect chromosomes from degradation and genetic information loss. Normal diploid cells lose telomeres with each cell cycle. Telomere length, therefore, decreases over time and may predict lifespan. Telomere shortening has negative effects on health conditions and has been linked to many health issues including aging and cancer. Accurate and consistent quantification of telomere length is important in many aspects of cell biology such as chromosomal instability, DNA repair, senescence, apoptosis, cell dysfunctions, and oncogenesis.

We offer 2 types of SYBR® Green qPCR-based assay kits for the accurate, simple and fast telomere length quantification for Human, Mouse and Rat samples.

FEATURES

Unique : specially modified primers
Accurate : highly specific and qPCR close to 100%
Reliable : No detectable primer dimer formation after 28-30 cycles
Informative : telomere length expressed in kilobases (Cat # 8918 only)
Sensitive : as low as 0.1ng of gDNA necessary
Rapid : results can be acquired within 1 hour
Robust : suitable for high-throughput setup

ABSOLUTE

With a reference gDNA sample with known telomere length included. This kit can be used to calculate a sample's actual telomere lenght in kb.

  • Absolute Human Telomere Length Quantification qPCR Assay Kit - 8918-SC
  • Absolute Mouse Telomere Length Quantification qPCR Assay Kit - M8918-SC
  • Absolute Rat Telomere Length Quantification qPCR Assay kit - R8918-SC

RELATIVE

NO reference gDNA sample included. Can be used to compare samples telomere length infold change.

  • Relative Human Telomere Length Quantification qPCR Assay Kit - 8908-SC
  • Relative Mouse Telomere Length Quantification qPCR Assay Kit - M8908-SC
  • Relative Rat Telomere Length Quantification qPCR Assay kit - R8908-SC



Figure 1. qPCR amplification curves using serially diluted telomere repeats as template.