Hepatitis G Virus Antibody (IgM) BioAssay™ ELISA Kit

Referencia 351647-96T

embalaje : 96Tests

Marca : US Biological

Contact local distributor :


Teléfono : +1 850 650 7790


Applications
E

Shipping Temp
Blue Ice

Storage Temp
4°C/-20°C

Sample Type:
Serum

Intended Use:
For the qualitative determination of human Hepatitis G virus antibody (IgM) concentrations in serum.

Specificity:
This assay has high sensitivity and excellent specificity for detection of human HGV antibody (IgM). No significant cross-reactivity or interference between human HGV antibody (IgM) and analogues was observed.

Test Principle:
This BioAssay™ employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Samples are pipetted into the wells with anti-human IgM conjugated Horseradish Peroxidase (HRP). Any antibodies specific for the antigen present will bind to the pre-coated antigen. Following a wash to remove any unbound reagent, a substrate solution is added to the wells and color develops in proportion to the amount of human HGV antibody (IgM) bound in the initial step. The color development is stopped and the intensity of the color is measured.

Kit Components:
Coated assay plate, 1x96 wells
Negative Control, 1x300ul
Positive Control, 1x300ul
Sample Diluent, 1x12ml
HRP-conjugate, 1x12ml
Wash Buffer (30X), 2x15ml
Substrate A, 1x6ml
Substrate B, 1x6ml
Stop Solution, 1x6ml

Storage and Stability:
Store powder at 4°C liquid at -20°C. Store other components at 4°C. Stable for at least 6 months For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Important Note
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.