Bead-based immunoassays and flow cytometry : Minimize reagent consomption - Maximize data quality
DropArray Technology for flow cytometry and Bead-based immunoassays
Technology and applications
How do wall-less plates work?
The DropArray plates are made with a hydrophobic planar surface and either 96- or 384- hydrophobic spots, arrayed in the standards SBS/ANSI format. DropArray plates are uniquely designed for three applications.
DA-Cell plates are used for cell processing of suspensions cells for flow cytometry assays. Each plate has 96 wells designed with two satellites to allow for dual-nozzle laminar flow washing via the DA-Cell washing station (HT1000). Each well has its own nozzles for quick and simultaneous washing of each well. Each well can have volumes up to 70 ul which is equivalent to approximately 5 million cells.
DA-Bead plates are used for Multiplex, Singulex, and other bead-based immunoassay assays. Plates are formatted in either 96- or 384-well formats and laminar flow washing occurs with by flooding the plate with buffer via the DropArray washing stations (LT210 or HT200).
Maximize Data Quality. Minimize Reagent Consumption.
DropArray technology has applications and product specific offerings in three main areas of research:
- Multiplex and other bead-based platforms such as Singulex SMC
- Cell processing for staining and retention of suspension cells in flow cytometry applications
Centrifuge-less cell processing for flow cytometry assays
Traditional centrifuge-based preparationof cells are time consuming, labor intensive, waste expensive reagents, and stress cells. There is significant cell loss and incomplete washing resulting in problems with gating and cell segmentation.
DA-Cell technology retains more than 95% of cells, completes washing in 2-4 minutes, significantly easier gating and segregation of cell populations even with high number of colors, and offers consistent, operator-independent, reliable data.
Bead-based immunoassays and Singulex SMC Assays
Traditional microplates require large volumes of reagent. Beads and assay reagents are expensive, limiting data output. The majority of beads, which are very costly, are wasted unnecessarily. The walls limit efficient washing and enable bead aggregation affecting the quality of data.
Miniaturization with the DA-Bead plates reduces sample volume and bead consumption by 5X and decreases assay costs by >60% without compromising quality or sensitivity. Laminar flow washing is efficient and limits bead aggregation resulting in superior data with challenging samples.
Uniquely Designed Features of Plates by Application
Suspension cell preparation for flow cytometry
Dual nozzole individual cell
Up to 70µl
Miniaturization of Multiplex, Singulex, and other bead-based assays
Protein detection / immunoassays
Whole plate washing
5 to 20 µl