MHC Tetramers

MHC Tetramers

The ONLY method to directly measure antigen-specific T cells.

20161007  

MHC tetramers are complexes of 4 MHC molecules (Major Histocompatibility Complex) wich are associated with a specific peptide and bound to a fluorochrome.

 

 

Class I tetramers bind to a distinct set of T cell receptors (TRCs) on a subset of T CD8+ cells, Class II tetramers bind to a distinct population of T CD4+ cells.

The patented MHC iTag tetramers reduce non-specific bindings to allow unique detection and enumeration of Ag specific T cells.

Thus, by mixing Tetramers with PBMCs or whole blood and using flow cytometry as a detection system, a count of all CD4+ or CD8+ T cells that are specific for one peptide and its matched MHC allele is provided, regardless of functionality.

CD1d tetramers allow NKT cells detection.

Advantages:

  • The only method to detect antigen specific CD4+ or CD8+ T cells ex vivo
  • Immune responses are targeted to specific allele
  • Tetramer staining is quantitative
  • Does not require the use of radioactive isotopes
  • Rapid: allowing fresh blood (or tissue-derived) sample to be analyzed
  • Large number of samples can be processed using flow cytometer
  • Tetramer-labeled cells remain viable
  • All specific CTLs are detected
  • Tetramers can be customized for unique applications


Specific Mutation reducing non specific binding:

The class 1 MHC present a proprietary mutation of HLA alpha 3 domain region which greatly reduces the non specific binding

Mutated tetramer
Conventional tetramer

 
Diseases:

  • Infectious diseases: VIH-SIDA, EBV, CMV, HPV, HBV, HCV, Influenza, Measles, Malaria, TB and RSV
  • Cancers: Breast, Prostate, Melanoma, Colon, Lung, Cervical, Ovairian et Leukemia
  • Autoimmunes diseases: Diabetes, Lyme disease, Multiple sclerosis, Rheumatoid arthritis, Autoimmun vitiligo
  • Transplantation: EBV and CMV


Alleles available:

Class I

Class II

CD1d
Human

Human

Human
HLA-A*01:01 HLA-A*29:02 HLA-B*40:06
DPB1*04:01
DRB1*04:05 DRB1*11:01
HLA-E*01:01 HLA-E*01:03
HLA-A*02:01 HLA-A*31:01 HLA-B*52:01
DRB1*01:01
DRB1*07:01 DRB1*15:01


HLA-A*02:06
HLA-B*57:01
DRB1*03:01
DRB1*08:03 DRB1*15:02
Mouse
HLA-A*02:07 HLA-B*07:02

DRB1*04:01
DRB1*09:01 DRB4*01:01
Qa-1b
HLA-A*03:01 HLA-B*08:01 HLA-C*03:03
Mouse alleles



HLA-A*11:01 HLA-B*15:01 HLA-C*03:04
IAb-OVA IAb-ESAT-6 IAb-MOG


HLA-A*23:01 HLA-B*27:05 HLA-C*08:01






HLA-A*24:02 HLA-B*35:01 HLA-C*12:02






HLA-A*26:01 HLA-B*40:01







Mouse







H2-Kb H2-Db H2-Ld






H2-Kd H2-Dd







H2-Kk H2-Dk







Mauritian Cyno







Mafa-A1*063 Mafa-B*104:01







Human-Mouse chimeras







A2Kb A24Kb







Rhesus Macaque






Mamu-A*01






Chicken







BF2*1201 BF2*1501








Custom products:

Developed to your:

  • allele
  • peptide
  • fluorochrome (PE or APC)
For more informations, please contact our technical service!